Introduction
R&D Systems Proteome ProfilerTM Human Cell Stress Antibody Array, Panel A (Catalog # ARY018) is a rapid, sensitive, and economical tool used to simultaneously detect the relative levels of 26 different cell stress-related proteins in a single sample. No specialized equipment is necessary.
- Product Insert
- Troubleshooting Guide
- Assays for analytes represented in the Human Cell Stress Array Kit
| Simultaneously detect the levels of these cell stress-related proteins in a single sample. | ||
| ADAMTS1 | HIF-2 alpha | Phospho-p38 alpha (T180/Y182) |
| Bcl-2 | Phospho-HSP27 (S78/S82) | Phospho-p53 (S46) |
| Carbonic Anhydrase IX | HSP60 | PON1 |
| Cited-2 | HSP70 | PON2 |
| COX-2 | IDO | PON3 |
| Cytochrome c | Phospho-JNK Pan (T183/Y185) | Thioredoxin-1 |
| Dkk-4 | NFkappaB1 | SIRT2 |
| FABP1/L-FABP | p21/CIP1 | SOD2 |
| HIF-1 alpha | p27/Kip1 | |
General Assay Principle
Carefully selected capture antibodies have been spotted in duplicate on nitrocellulose membranes. Cell or tissue lysate samples are diluted and mixed with a cocktail of biotinylated detection antibodies. The sample/antibody mixture is then incubated with the array. Any protein/detection antibody complex present is bound by its cognate immobilized capture antibody on the membrane. Streptavidin-Horseradish Peroxidase and chemiluminescent detection reagents are added, and a signal is produced in proportion to the amount of cytokine bound. Chemiluminescence is detected in the same manner as a Western blot.
Kit Contents
- 4 Array Membranes
- 4-Well Multi-dish
- Array Buffers
- Lysis Buffer
- Wash Buffer
- Detection Antibody Cocktail
- Streptavidin-HRP
- Chemiluminescent Detection Reagents
- Transparency Overlay Template
- Detailed Protocol
For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.